Purebloods (純血種, Junketsushu) are the highest rank among vampires classed as Level A (Aクラス, A Kurasu, lit. "A Class"). They are "pure" vampires without any human ancestry, and can trace their lineage back to the original vampire progenitors.
Purebloods all possess a series of unique powers, often exclusive only to the certain Pureblood or Pureblood family.
A very long time ago, when the human race was on the verge of extinction the first vampires emerged. These first vampires, known as Purebloods, appeared. Purebloods are those who have not bred with humans, thus keeping their vampire blood pure. Some Purebloods breed with Humans producing weaker vampires which have came to be known as the Noble vampires. Most vampires have some human blood in them, but a Pureblood vampire does not. Purebloods, like all vampires are sensitive to the sun as it only hurts their eyes and can get suburned easily.
War broke out between the Purebloods and the Humans at some point a long time ago. In order to defend themselves against vampires, some humans ate an ancestor's flesh, creating the vampire hunters which granted them the power and strength to kill vampires. This includes anti-vampire magic and weapons. Therefore all vampire hunters have vampire blood in them but not to the extent that they are classified as vampires, thus they do not benefit from a vampires longevity and thirst for blood. The vampire hunters protect the Human race from the vampires.
During the war, the Pureblood population greatly diminished. Many Purebloods drank the blood of humans during this time thus creating Level E vampires to increase the vampire population. Consequently, there are few Purebloods today.
The full extent of their powers are only known amongst their Pureblood companions. Their true powers remain a mystery. They have ethereal beauty of the Purebloods. They are immortal, meaning they live forever unless an anti-vampire weapon is used to cut off their heads or pierce their hearts. Any other injury is healable, including having one's head cut off or blown to pieces, if a Pureblood uses the blood of another Pureblood to heal them. When a Pureblood vampire is killed, their bodies slowly disperse into small sparkles before shattering into shards of glass.
It is heavily implied that each vampire has a unique power such as Rido Kuran's ability to manipulate his blood or Shizuka's control over plants. Their blood is highly prized and many have attempted to drink their blood, this includes both humans and Noble vampires. The blood of a Pureblood can give vampires new powers. Aside from the standard powers shown by other vampires, Purebloods have also demonstrated the following:
- They can form shadow puppets/spies using parts of their flesh which are capable of shape shifting.
- They can transform parts of themselves into animals. 
- Purebloods can create familiars of any form they choose, use their powers and control them.
- Powers to perform spells to seal off the vampire side of vampires, thus making them a normal human. The spell also erases all of his/her memories of being a vampire. However this spell comes at the price of their life. (The vampire converted to human, eventually must return to his/her true nature, if not, he/she descends into madness. However, this was only in Yuki's case as Isaya said his son died human).
- Ability to control lower vampires physically.
- Ability to put vampires and humans to sleep.
- Erasing Memories and can control elements.
- Telekinesis and can cast spells.
- Morph the flesh and bodies of others transforming them.
- Ability to possess the bodies of other vampires.
- They can also put themselves to sleep for a very long time, even hundreds of years, and be resurrected through a blood sacrifice.
- Purebloods can become each other's masters if one is awakened by another from slumber.
- Enhanced sense of smell.
- Ehanced strength, speed, and stamina.
- Enhaced durability, agility, and senses.
- They can feel and see memories of others by drinking their blood.
- Their blood can heal human illnesses and give them a small amount of strength. (But for a human to drink pureblood's blood, might die due to their blood being highly poisonous to them).
- Ability to turn humans into vampires and retain complete control over them by becoming their master, rendering the vampire unable to kill them. The blood of their master will also prevent an ex-human from becoming a Level E vampire.
The Kurans are unique among Purebloods, as some members of the family possess and display the ability to handle anti-vampire weapons. Juri Kuran, Yuki Kuran, Kaname Kuran (although he can hold such weapons, it does harm him) and Rido Kuran have demonstrated this ability.
Relationships between Purebloods
Purebloods tend to not show emotion (Yuki Kuran is an exception to this as she had lived as human, and thus doesn't want to bottle up her feelings) and are wary of other Purebloods.
The Pureblood nature is an everlasting quest for power. By killing and drinking the blood of another Pureblood, they can absorb their power. However, this is a great taboo and is said to summon a sinister future upon that Pureblood, unless it is their lover. Similar to other vampires, a Pureblood's blood thirst can only be quenched by the blood of their loved one. Since Purebloods live for eternity, losing their loved one is much more devastating for them.
It is also common practice for Pureblood siblings to marry each other to preserve their thick blood. For example, the Kuran family, who are at the core of the vampire world. Purebloods are the monarchy of the vampire race with the Kuran family serving as the Queens and Kings.
Relationships between other vampires
Relations between the Purebloods and the Nobles are mixed. Noble vampires who are Pro-Monarchy have a strong sense of loyalty to Purebloods as seen by Aido who refers to Kaname as "Kaname-sama" (Respectful and formal Japanese term) and always protects Yuki. It is very important for Purebloods to continue the vampire race, therefore many Noble children are put forward as potential wives or husbands. As opposed to other vampires who are Pro-Vampire Council view the Purebloods as a threat because of their great power; for example Asato Ichijo wanted to rid the world of Purebloods because they have the power to absolutely control lesser vampires will. However, no matter what a vampire's political views are, they all have a seemingly instinctual fear and awe for Purebloods. The Purebloods and the Vampire Council also work together in order to maintain order in the vampire society by keeping watch over Level Ds and exterminating Level Es.
List of Pureblood Vampires
The Kuran family
The Hio Family
The Shirabuki Family
The Shoto Family
The Ori Family
The Toma Family
- Shizuku Toma
- Unknown Female
The Hanadagi Family
- Given Purebloods usually marry their siblings and have produced offspring without problem for centuries, they obviously lack the human weakness of needing a versatile gene pool.
- While purebloods originally stop aging in their mid-twenties, putting themselves into slumber can alternate their age.
- Purebloods pregnancy can last 2 to 5 years and can increase their thirst for human blood. When purebloods become pregnant they stay away from humans to prevent feeding from them.
- Pureblood vampire children do not drink blood until they have their fangs. Like all vampires, young pureblood children take energy until they have to drink blood. When purebloods gets their fangs they have to stay inside their home until they gain control of their impulse to drink blood.
- Pureblood's blood is desired more than the blood of human children and the blood of their beloved.
- Purebloods, similar to their Ancestor are the progenitors of the vampire race, with Kaname Kuran being the ancestor and head of the kuran family.
- When purebloods crave blood, they will feel a burning pain in their throat or use their powers their eyes glow red. Purebloods can quench their thirst for blood through blood tablets and transfusion blood bags.
- Besides turning into a vampire, when a pureblood bites a human, the human will die from blood loss and poisoning. While pureblood blood is very powerful it can be dangerous to vampires and humans.
- ↑ The Night Class students attends school at evening/night to avoid the sun and Rima Toya can be seen carrying a parasol.
- ↑ Yuuki Kuran can transfrom into butterflies, while Kaname Kuran can transform into bats.
- ↑ Yuki Kuran can create familiars of butterflies, Kaname Kuran can create famaliars of bats and a wolf, and Sara Shirabuki can create familiars of spiders.
- ↑ Kaname Kuran used this power against a Ex-Human Vampire.
- ↑ Kaname Kuran does this to Yuki.
- ↑ Juri Kuran's power.
- ↑ Both Kaname Kuran and Yuki Kuran display this ability.
- ↑ Haruka Kuran use this ability on Rido Kuran's vampire servants.
- ↑ Shizuka Hio possesses Maria Kurenai's body.
- ↑ Rido Kuran possesses Senri Shiki's body.
- ↑ Ai Kuran and Kaname Kuran put themselves into slumber once.
- ↑ Rido Kuran does this when he resurrects Kaname Kuran.
- ↑ The vampires of the Night Class are able to smell blood when Zero bites Yuki, even they they aren't nearby.
- ↑ Shizuka Hio turned Zero Kiryu into a vampire, which also prevented him from killing her.
- ↑ Since Kaname Kuran is the head of the kuran family, he is the pureblood king. While Yuki Kuran is the pureblood princess.